Fig. 7: Human ILC2s express DR3, and this expression is inducible by IL-33.

a The gating strategy of Lin^-CD45⁺CD127⁺CRTH2⁺ human ILC2 cells. b Human peripheral-blood ILC2s were freshly sorted and cultured with 10 ng/mL of recombinant human (rh) IL-2 and rhIL-7 in presence or absence of rhIL-33 (10 ng/mL) for 12, 24, and 48 h. Freshly isolated ILC2 at 0 h and ex vivo activated ILC2s were analyzed by flow cytometry as indicated in the scheme, and (c) the kinetics of DR3 induction by IL-33 is shown. d The levels of IL-5, IL-6, IL-8, IL-9, IL-13, and GM-CSF in the culture supernatants were measured by Luminex after 48 h of stimulation. Data representative of five individual blood donors, n = 5. Error bars are the mean ± SD. Statistical analysis, one-way ANOVA (c) or two-tailed student’s t-test (d); n.s.: p-value of <0.05 was considered as non-significant. Human image provided with permission from Servier Medical Art.