Fig. 4: Genetic variation in OsNramp5 in Luohong3A and Luohong4A.
a Deletion that spans OsNramp5 (marked by the red arrow) in Luohong3A and Luohong4A shown using JBrowse52 (v1.12.3). R93-11, a commonly used 2R, was used as a control to demonstrate no large deletion. b Gene structure of OsNramp5 in Nipponbare showing the location of regions used for PCR: C1 on exon 1, C2 on exons 7 and 9, and C3 on exon 13. c Agarose gel electrophoresis of PCR products for accessions R93-11 (positive control), Nipponbare (positive control), Luohong3A, and Luohong4A using three pairs of primers targeting the C1, C2, and C3 regions shown in b. The rice actin gene served as the PCR DNA control. The marker sizes (bp) are shown on the left side. Three biological replicates were analyzed for each sample and a representative gel image is shown. d Bar plot for Cd content in leaves (dark) and roots (white) of Huazhan (positive control), Huazhan-OsNramp5 (OsNramp5 knockout39, negative control), Luohong3A, and Luohong4A. Mean values ± s.d. (n = 3) are shown. All individual data points are shown as dots. Source data underlying c and d are provided as a Source Data file.
