Fig. 4: Heat-inactivation of SARS-CoV-2 confirmed by plaque assay.
From: Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR
Box plots showing the number of plaque forming units (PFU) observed after heat inactivation (95 °C 5 min, left) of in vitro propagated active SARS-CoV-2 as well as for a dilution series of SARS-CoV-2 without heat inactivation (right), with circles indicating the values of individual replicates. Center lines denote the median, hinges denote IQR and whiskers denote outlier points at maximum 1.5 × IQR. Virus was added to 9.6 cm2 dishes seeded with 1 million Vero E6 cells and plaque assays were performed as described in “Methods”. Undiluted samples contained ~2.5 million PFU of SARS-CoV-2. Representative images are shown below each condition (scale bar 5 mm).
