Fig. 6: Improved detection of COVID-19 in a lateral flow assay using ENHANCE. | Nature Communications

Fig. 6: Improved detection of COVID-19 in a lateral flow assay using ENHANCE.

From: Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection

Fig. 6

a Schematic diagram showing how a lateral flow assay works. Briefly, the dipstick uses gold-labeled FITC-specific antibodies that bind to FITC-biotin reporters and travel through the membrane. Only the cleaved reporters will reside at the positive line. b Lateral flow assay detecting SARS-CoV-2 RNA N gene using crCov-2 and crCoV-2 + 3′DNA7 with RT-LAMP, and c band-intensity analysis of b using ImageJ. d Lateral flow assay detecting SARS-CoV-2 cDNA using crCov-2 and crCoV2 + 3′DNA7 without a pre-amplification step, and e band-intensity analysis of d using ImageJ. Source data are available in the Source Data file.

Back to article page