Fig. 7: Enhanced sensitivity and specificity with ENHANCE for detecting SARS-CoV-2 genomic RNA. | Nature Communications

Fig. 7: Enhanced sensitivity and specificity with ENHANCE for detecting SARS-CoV-2 genomic RNA.

From: Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection

Fig. 7

a Sequence alignment of similar pathogens from the same family with SARS-CoV-2 that were tested in this study. Two crRNAs including their engineered version were designed to target two regions of the N gene N1 and N2 (N1: crCoV and N2: crCoV) where N2 was reported in Broughton et al. Sequences were aligned using ClustalOmega31,32, exported in aln file and graphically enhanced in ESPript 3.033. b crRNA specificity towards SARS-CoV-2 and other highly similar pathogens from the same family. The targets were dsDNA amplified from plasmid controls 2019-nCoV_N_Positive Control, MERS-CoV Control, and SARS-CoV Control (IDT). c Detection reaction in b scanned by Typhoon (Amersham, GE healthcare). d crRNA specificity towards genomic RNA of SARS-CoV-2 and other genomic RNAs of highly similar pathogens from the same family. The targets were genomic RNA obtained from BEI Resources. e Lateral flow assay of d. f Detection reaction in d scanned by Typhoon (Amersham, GE healthcare). For b, and d, error bars represent mean ± SEM, where n = 6 replicates (three technical replicates examined over two independent experiments). Source data are available in the Source Data file.

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