Fig. 2: Evaluation of sTyrRS in mammalian cells.
From: Functional genetic encoding of sulfotyrosine in mammalian cells
a Confocal images of 293T cells expressing the evolved sTyrRS and an EGFP mutant that contained an amber mutation at position Tyr40. The left panel shows fluorescent images, the middle panel shows brightfield images, and the right panel shows composite images of bright-field and fluorescent images. Scale bars, 50 μm. b Flow cytometry analyses of 293T cells expressing the evolved sTyrRS, AzFRS, and wild-type EcTyrRS, each with the EGFP-Y40TAG mutant. The normalized fluorescence was calculated by multiplying the mean fluorescence intensity by the percentage of fluorescent cells in flow cytometry analyses in the absence (open bar) or presence (filled orange bar) of ncAA. b Data are plotted as the mean ± s.d. from n = 3 independent experiments. The raw data of flow cytometry experiments are shown in Supplementary Fig. 2. b Source data are provided as a Source data file.
