Fig. 4: Imaging of a cubic millimeter of mouse cortex with piTEAM. | Nature Communications

Fig. 4: Imaging of a cubic millimeter of mouse cortex with piTEAM.

From: A petascale automated imaging pipeline for mapping neuronal circuits with high-throughput transmission electron microscopy

Fig. 4

a Scaling from 0.003 mm3 to 1 mm3, a 300-fold volume increase; b High-resolution electron microscopy image tile from the 1 mm3 dataset with neuronal somata highlighted in yellow and glia in green (scale bar 5 µm). c Zoomed-in area from (b) showing synapse with dendritic spine (scale bar 1 µm); d Zoomed-in area from (b) showing synapse with dendritic shaft (scale bar 1 µm); e Low-mag EM image of an aperture with an ROI highlighted; 2D stacked montage minimap and aligned 3D volume. f Distribution of montage acquisition rate (frames per second) achieved during 1 mm3 production. The plots represent a sample size of over 1000 sections imaged by 20 Mpixel and 50 Mpixel cameras each. g Example of stage step-and-settle time distribution for GridStage.

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