Table 1 Performance metric during 1 mm3 production for 20 MP and 50 MP cameras.

From: A petascale automated imaging pipeline for mapping neuronal circuits with high-throughput transmission electron microscopy

Imaging parameters

Avg. production 20 Mpixel

Avg. production 50 Mpixel

50 Mpixel peak

Unit

Frame size (pix)

3840

5408

5504

Aspect ratio of 1:1

Resolution

3.95–4

3.95–4

4

nm/pixel

Tile overlap

13

9

9

%

Net imaging rate

3.2–3.5

3.2–3.5

4.0

Hz

FOV per side

15

21

22

µm

AOV

225

468

484

µm2

Pixels per image

14.7

29.2

30.3

Mpixel

Time per section

30–40

15–25

~14

Minute

Tiles w/overlap

5100

2916

2600

Per section

Sections per week (24/7)

~300

~490

~700

Section per week

Time to image volume with one scope @65% uptime

900

525

450

Day

Time to image volume with five scopes @65% uptime

180

105

90

Day

Completion of project in months @65% w/5 scopes

~6

~3.5

~3

Month

  1. An estimation of mean 65% uptime takes into account the downtime from microscope maintenance, barcode reading errors, and imaging pause determined by real-time QC.