Fig. 4: Relationship between pKa and pH with the activity of rBxTW1-E495A. | Nature Communications

Fig. 4: Relationship between pKa and pH with the activity of rBxTW1-E495A.

From: Thioglycoligase derived from fungal GH3 β-xylosidase is a multi-glycoligase with broad acceptor tolerance

Fig. 4

a Yield of acceptor conversion (%) into a xylosyl derivative in the presence of donor excess. The initial donor/acceptor ratio was 2/1 (mole/mole) and the acceptor conversion was determined by HPLC at different time intervals for 4 h. b Ligase activity response assay for each acceptor. The effect of each acceptor on the activity of rBxTW1-E495A was assayed over a range of acceptor concentrations. β-Xylosidase activity was determined spectrophotometrically using pNPX as substrate. Determined profiles are represented by a dashed line and black circles for pH 2.2, by a continuous line and white circles for pH 3.0 and by a continuous line and black circles for pH 5.5. Mean values are shown together with the corresponding standard error (n = 3 independent experiments). Source data are provided as a Source Data file.

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