Fig. 5: Production of different xylosides with and without pH control. | Nature Communications

Fig. 5: Production of different xylosides with and without pH control.

From: Thioglycoligase derived from fungal GH3 β-xylosidase is a multi-glycoligase with broad acceptor tolerance

Fig. 5

The plots present the acceptor conversion (%) over time, determined by HPLC, using a 2/1 (mole/mole) donor/acceptor balance for every acceptor, except for phosphoric acid. The latter was analyzed by NMR and yield was calculated in terms of donor conversion (%) using a 1/2 (mole/mole) donor/acceptor balance. Yield profiles are represented by a dashed line and black circles for non-controlled pH and by a continuous line and white circles for controlled pH. Mean values are shown together with the corresponding standard error (n = 3 independent experiments) for every acceptor except phosphoric acid. Formation of the xylosyl phosphoester was assayed with a single replicate by continuously measuring the corresponding sample over time. However, due to the testing of multiple timepoints and two different conditions, results are still significant to verify the formation and subsequent consumption of the product. Source data are provided as a Source Data file.

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