Fig. 3: Copy number of antimicrobial resistance genes present on the translocatable unit increased following in vitro replication of the evolutionary event leading to piperacillin/tazobactam resistance. | Nature Communications

Fig. 3: Copy number of antimicrobial resistance genes present on the translocatable unit increased following in vitro replication of the evolutionary event leading to piperacillin/tazobactam resistance.

From: Piperacillin/tazobactam resistance in a clinical isolate of Escherichia coli due to IS26-mediated amplification of blaTEM-1B

Fig. 3

Fold change in copy number of all the antimicrobial resistance genes found on the pseudo-compound transposon compared to the housekeeping gene uidA following growth of the piperacillin/tazobactam (TZP)-susceptible isolate in the absence of antibiotics (pink circles), TZP-susceptible isolate in the presence of 8/4 µg/ml TZP (black circles), TZP-susceptible isolate transformed with pHSG396 plasmid containing IS26 in the presence of 8/4 µg/ml TZP and 35 µg/ml chloramphenicol (green circles) and TZP-susceptible isolate transformed with pHSG396 plasmid containing IS26 in the presence of 35 µg/ml chloramphenicol only (purple circles). All error bars represent the standard error of the mean (n = 3). Source data are provided as a Source Data file.

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