Fig. 3: mRNA dynamics of indicated genes during MZT in human.

a RT-qPCR results showing the mRNA levels of selected M-decay transcripts at the GV, MII, and zygote (3PN) stages. b and c RT-qPCR results showing the mRNA levels of selected Z-decay transcripts at the indicated stages. Data are presented as mean values ± SEM. P by a two-tailed Student’s t-test. n = 3 biological replicates. d and e The RNA-seq results showing the levels of transcripts encoding key factors of the M-decay d and Z-decay e pathways in human oocytes and early embryos. FPKM values were extracted from previously published data (GSE36552²⁸). f and g Immunofluorescence results showing the protein levels of BTG4, CNOT7, and YAP in human oocytes, zygotes containing three pronuclei (3PN), and 8-cell embryos. Scale bars = 40 µm. Immunostaining of each antibody was independently repeated for three times with similar results.