Fig. 6: Transcriptome analyses for developmental arrested human zygotes after IVF.

a The principal component analysis (PCA) results of developmental arrested zygotes 3 days after IVF. TUBB8^mutant indicates zygotes of TUBB8-mutated patients; unid-patient indicates mutation-unidentified patients. b A scatter plot is shown, which compares transcripts in arrested zygotes that were derived from TUBB8-mutated and unid-patients. Transcripts decreased or increased more than 2 folds in unid-patient samples compared to TUBB8^mutant samples, which are highlighted in blue and red, respectively. n gene number, FC fold change; 1–5, embryos from different unid-patients. c Heatmap of genes upregulated or downregulated more than 2 folds in zygotes from unid-patients compared to TUBB8^mutant samples. The definition of Groups A and B is described in the text. d A scatter plot is shown, which compares transcripts in arrested zygotes that were derived from TUBB8-mutated and unid-patients. Transcripts decreased or increased more than 5 folds in zygotes of unid-patients compared to TUBB8^mutant samples, which were highlighted in blue and red, respectively. n gene number; FC fold change; 1–5, represent embryos from different unid-patients. e Numbers of transcripts that were upregulated (red) or downregulated (blue) in arrested zygotes derived from unid-patients compared to those derived from TUBB8-mutated patients. f Venn diagram showing the overlap of upregulated transcripts in arrested zygotes of unid-patients and the degraded transcripts from the GV-to-zygote transition in normal oocytes. P = 1e−122 by a two-tailed Student’s t-test. g Heatmap showing the levels of M-decay transcripts (downregulated more than 2 folds from the GV stage to the zygote stage in normal samples (FPKM (GV) + 1/FPKM (zygote)+1) >2)) in arrested zygotes from TUBB8-mutated patients and unid-patients. h Gene ontology analysis of transcripts upregulated or downregulated more than 2 folds in arrested zygotes derived from unid-patients compared to those from TUBB8-mutated patients.