Fig. 6: CLR01 protects dopaminergic neurons from LB-induced cell death through reduction of α-syn aggregation in mice.
From: CLR01 protects dopaminergic neurons in vitro and in mouse models of Parkinson’s disease
a, b Representative photomicrographs of TH-immunostained SNc and striatum (respectively) in LB-inoculated mice after osmotic mini-pump implantation for CLR01 (or PBS) delivery (40 µg/kg/h). c Stereological cell counts of SNc TH-immunoreactive neurons (a) in LB-inoculated mice, at 3 months post-LB inoculation. p = 0.0177. d Optical densitometry of striatal TH immunoreactivity in LB-inoculated mice (b), at 3 months post-LB inoculation. p = 0.0354. e, g Representative photomicrographs of α-syn and PK-resistant α-syn immunostained SN from LB-inoculated mice, and corresponding quantification. f, h Representative images of Syn-F1 immunostaining in the SN in LB-inoculated mice and corresponding quantification of the intensity levels of Syn-F1 staining. f p = 0.0333, h p = 0.0556. a–h n = 4–5 for LB-inoculated mice, n = 5–7 for LB + CLR01-independently treated animals, analyzed with a two-tailed Mann–Whitney U-test. *p < 0.05. Scale bars = 0.1 mm (SN) and 1 mm (Str). For all appropriate panels, data are presented as mean values ± SEM.
