Fig. 3: Evaluation and classification of the 1-based OBOC library.

a The scatter plot for the three different assays of the bead-derived 13,584 peptide solutions. H⁺/Na⁺ transport activities of the peptides were normalized against the activity of 1. Cell viability (%) was evaluated using the P388 mouse leukemia cell line. Antibacterial activity against S. pyogenes was evaluated using the three concentrations and the potency is coded by three colors. +++ (red square): inhibition by the 640-fold diluted peptide solution, ++ (yellow square): inhibition by the 160-fold diluted peptide solution, + (cyan square): inhibition by the 40-fold diluted peptide solution, - (navy square): no inhibition. The dots denoting the parent 1 are indicated by the purple circles. The dots that did not meet any criteria are shown as gray filled circles. Source data are provided as a Source Data file. b Distribution percentages of the randomized four amino acids and percentages of the active peptides against S. pyogenes. Amino acids are displayed as one-letter codes (Asm = N′). Compounds were defined as active if one or more solutions was active in the antibacterial assay. c Selection criteria of the 10 peptides (A1, B₀1–B₀4, B₁1–B₁3, and B₂1–B₂2) from groups A and B.