Fig. 3: Functional verification of the oxepinase OpaB. | Nature Communications

Fig. 3: Functional verification of the oxepinase OpaB.

From: Oxepinamide F biosynthesis involves enzymatic d-aminoacyl epimerization, 3H-oxepin formation, and hydroxylation induced double bond migration

Fig. 3

a HPLC analysis of the conversion of 3 to 4 in the opaB expression strain of A. nidulans at 254 nm. b A sequence logo for the conserved EXXR motif in OpaB using sequences of 96 P450 enzymes (Supplementary Table 9). c Proposed mechanism for oxepin formation catalyzed by OpaB (SH: substrate).

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