Fig. 5: In vitro verification of OpaE as a d-phenylalanyl epimerase. | Nature Communications

Fig. 5: In vitro verification of OpaE as a d-phenylalanyl epimerase.

From: Oxepinamide F biosynthesis involves enzymatic d-aminoacyl epimerization, 3H-oxepin formation, and hydroxylation induced double bond migration

Fig. 5

a HPLC analysis of in vitro assays with OpaE at 254 nm. b SDS-PAGE analysis of the purified OpaE. The experiments were repeated twice. c Determination of the kinetic parameters for OpaE with 5.The error bars represent standard errors of velocity and SEMs are given as ± values (n = six independent experiments). d LC–MS monitoring of the OpaE reactions in D2O/H2O and negative controls. e Proposed mechanism for the OpaE reaction.

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