Fig. 1: PINCH-1 level is increased in lung adenocarcinoma.

a, b Human lung adenocarcinoma tissues and adjacent normal tissues from a microarray were stained with anti-PINCH-1 (P1) antibody (a). The samples in columns 1, 3, 5, 7, 9, 11, 13, 15, and 17 on rows a–i and columns 1, 3, 5, 7, 9, 11, and 13–18 on row j were derived from lung adenocarcinoma tissues. All other samples were derived from adjacent normal tissues. Bar = 1 mm. The mean intensities (arbitrary units) of PINCH-1 staining from lung adenocarcinoma tissues (n = 93) and normal adjacent tissues (n = 87) were quantified (b). c Higher magnification images of representative IHC staining shown in panel a obtained with anti-PINCH-1 antibody (brown) and haematoxylin counterstain (blue). Bar = 20μm. The boxed areas in the IHC staining were enlarged and shown in the upper right corner. d Kaplan–Meier survival curve of 90 lung adenocarcinoma patients. Patients were divided into two groups according to the mean intensities of PINCH-1 staining in cancer tissues of the tissue array (high expression: n = 57, low expression: n = 35, Log-rank (Mantel–Cox) test was used for the statistical analysis). e Lung adenocarcinoma was induced by administration of Ad-Cre into the lung of Kras^LSL−G12D/+ mice. Sixteen weeks later, sections from the Kras^LSL−G12D/+ mice administrated with Ad-Cre or without Ad-Cre as a control were analyzed by immunostaining with anti-PINCH-1 antibody. Bar = 20 μm. The boxed areas in the IHC staining were enlarged and shown in the upper right corner. The mean intensities of PINCH-1 staining in lung adenocarcinoma were quantified and compared to those of normal lung tissue (normalized to 1; normal group n = 36 fields from 6 mice, cancer group n = 49 fields from 6 mice; P < 0.0001) (e, right panel). Data in e represent mean ± SEM. Statistical significance was calculated using two-tailed unpaired Student’s t-test, ***P < 0.001. Source data are provided as a Source Data file.