Fig. 3: PINCH-1 regulates mitochondrial fragmentation and H1299 cancer cell proliferation.
From: PINCH-1 regulates mitochondrial dynamics to promote proline synthesis and tumor growth
a H1299 lung adenocarcinoma cells were infected with PINCH-1 shRNA (Sh-P1) or control RNA (Sh-con) lentivirus, cultured for 5 days and analyzed by Western blotting with antibodies recognizing PINCH-1 (P1) or tubulin. b The cells were cultured for 4 days and the numbers of live cells were analyzed as in Fig. 2b (n = 4; H1299 vs Sh-P1 P < 0.0001, Sh-con vs Sh-P1 P < 0.0001). c, d The cells were stained with DAPI (blue) and antibody for Ki67 (purple) (d). Bar, 25μm. The percentages of Ki67-positive cells were quantified (e, n = 30; H1299 vs Sh-P1 P < 0.0001, Sh-con vs Sh-P1 P < 0.0001). e, f Mitochondria were stained with MitoTracker Red CMXRos (f, left panels) and the percentages of mitochondria with different morphologies were quantified (f, right panel, H1299 n = 33 cells, Sh-con n = 48 cells, Sh-P1 n = 56 cells; H1299 vs Sh-P1 P < 0.0001, Sh-con vs Sh-P1 P < 0.0001). Bar, 5 μm. Mitochondrial areas in z-stack images were quantified (e, n = 30 cells; H1299 vs Sh-P1 P < 0.0001, Sh-con vs Sh-P1 P < 0.0001). Data in b, c, e, and f represent mean ± SEM. Statistical significance was calculated using one-way ANOVA with Tukey–Kramer post-hoc analysis. ***P < 0.001. Source data are provided as a Source Data file.
