Fig. 3: TASOR lies at the heart of the HUSH complex.
From: TASOR is a pseudo-PARP that directs HUSH complex assembly and epigenetic transposon control
a TASOR interacts separately with MPP8 and Periphilin to mediate assembly of the HUSH complex. Pairwise combinations of HUSH subunits were exogenously expressed in HUSH triple KO cells, and interactions detected by co-immunoprecipitation (co-IP) followed by immunoblot. b TASOR DomI mediates the interaction with MPP8. The indicated TASOR truncation mutants were exogenously expressed in TASOR KO cells and their association with endogenous MPP8 assessed by co-IP. c SEC-MALS of recombinant TASOR(354–633)-MPP8(528–860) complex (theoretical MW, 72 kDa for a 1:1 complex) with representative SDS-PAGE gel of SEC peak fractions (inset). d The central linker of TASOR mediates the interaction with Periphilin. e Summary of core HUSH interaction sites.
