Table 1 Cryo-EM data collection, refinement, and validation statistics of PF846-stalled termination complexes.
From: Selective inhibition of human translation termination by a drug-like compound
Classical RNC (EMD-22085, PDB-6XA1) | Rotated RNC (EMD-22086) | |
|---|---|---|
Data collection and processing | ||
Magnification | 43,478 | 43,478 |
Voltage (kV) | 300 | 300 |
Electron exposure (e–/Å2) | 50 | 50 |
Defocus range (μm) | −1.0 to −2.0 | −1.0 to −2.0 |
Pixel size (Å) | 1.15 | 1.15 |
Symmetry imposed | C1 | C1 |
Initial particle (no.) | 183,759 | 183,759 |
Final particle (no.) | 57,324 | 26,463 |
Map resolution (Å) | 2.7 | 2.9 |
FSC threshold | 0.143 | 0.143 |
Refinement | ||
Average resolution (Å) | 2.7 Å (60 S)/2.8 Å (40 S)/2.8 Å (40S_eRF1_tRNA_mRNA) | 2.9 Å (60 S)/3.1 Å (40 S)/3.1 Å (40 S_tRNA_mRNA) |
Map-sharpening B factor (Å2) | −43 | −48 |
Initial model used (PDB code) | 6OLE | |
40S subunit | 6qzp | |
60S subunit | 6qzp | |
eRF1_tRNA_mRNA | 6d90 | |
R.m.s. deviations | ||
Bond lengths (Å) | 0.014 | 0.013 |
Bond angles (°) | 1.103 | 1.267 |
Validation | ||
Clashscore | 3.36 | 4.70 |
Poor rotamers (%) | 0.53% | 0.94% |
Ramachandran statistics | ||
Favored (%) | 93.85% | 92.46% |
Allowed (%) | 6.03% | 7.38% |
Outliers (%) | 0.53% | 0.17% |
