Table 1 NMR data collection and refinement statistics for the final set of 25 accepted structures.

	Total	VPS29	VARP
Structural restraints
NOE-derived distance restraints
Intraresidue		33	39
Sequential		22	40
Medium (2 ≤ \|i-j\|≤4)		24	17
Long (\|i-j\| > 4)		40	2
Total		119	98
Intermolecular	49
Dihedral angle restraints
χ¹		None	11
Template non-crystallographic symmetry restraints for VPS29 (to pdb 2R17)
Backbone
Strong^a	Residues 7–19, 60–148
Medium^b	Residues 1–6, 20–59, 149–181
Sidechain
Medium^b	Residues 1, 3–6, 20–24, 26, 28–29, 31–59, 149–151, 153, 155–160, 162, 164, 166–171, 173, 175–181
Weak^c	Residues 2, 25, 27, 30, 152, 154, 161, 163, 165, 172, 174
Structural statistics for accepted structures
Number of accepted structures	25
Mean XPLOR-NIH energy terms (mean ± s.d., kcal.mol⁻¹)
E(total)	−1694.0 ± 23.1
E(van der Waals)	175.8 ± 4.3
E(NCS)^d	153.8 ± 14.2
E(distance restraints)	39.8 ± 8.3
Restraint violations (mean ± s.d.)
Distance (Å)	0.081 ± 0.065
Dihedral angles (°)	0.99 ± 0.50
Max. distance restraint violation (Å)	0.646
Max. dihedral angle violation (°)	2.00
RMS deviations from the ideal geometry
Bond lengths (Å)	0.0024
Bond angles (°)	0.68
Improper angles (°)	0.51
Average pairwise atomic rmsd (±s.d.)
(N, C^α, C’ atoms)		0.10 ± 0.03 Å^e	0.33 ± 0.12 Å^f
(All heavy atoms)		0.21 ± 0.04 Å^e	0.69 ± 0.15 Å^f

^aForce constant 100.0 kcal mol⁻¹.
^bForce constant 2.0 kcal mol⁻¹.
^cForce constant 0.1 kcal mol⁻¹.
^dNCS energy terms are reported for the structures prior to addition of the unstructured tails of VPS29; no NCS terms are active during the final stage of the calculation when these tails are added.
^eResidues 1–181.
^fResidues 710–721.

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