Fig. 6: Inhibition adhesion assays of polyclonal antibodies generated against constructs of the P1 C-terminal domain.

a Residues corresponding to the P1 constructs used as antigens to generate polyclonal antibodies are colored in cyan in the structure of P1. The short construct (left, residues Ala1400–Asp1521) includes only the C-terminal domain. The long construct (right, residues Lys1376–Asp1521) includes also the hinge, which connects the N-terminal domain with the C-terminal domain, and the two strands from the last propeller β-sheet, also known as the cytoadherence epitope. b Sera raised against any of the two constructs inhibit adhesion of M. pneumoniae cells even at low concentrations, with 3% showing a significantly higher inhibition than 1%. Error bars represent the mean ± standard deviation. *p < 0.05 and ***p < 0.001. Source data are provided as a Source Data file.