Fig. 2: Kinetics of septin assembly imaged by HS-AFM.
From: The hierarchical assembly of septins revealed by high-speed AFM
a In situ HS-AFM frames in pH 5.8, 600 mM KCl (top left: imaging conditions; bottom left: image acquisition times) of the septin filaments assembly process. Filament alignment and pairing (green arrows) are established simultaneously. b In situ HS-AFM frames in pH 7.5, 150 mM KCl (top left, imaging conditions; bottom left: image acquisition times) showing a much slower assembly process resulting in shorter and less well aligned filaments. c Filaments higher-order analysis for (top: a) and (bottom: b), respectively. Data are presented as mean ± s.d. d Surface coverage as a function of time under varying conditions (150 mM or 600 mM KCl in pH 5.8 or pH 7.5 buffer). The solid lines are fitted sigmoidal curves. e, f Length histograms of septin assemblies as a function of time under conditions a shown in e, and b shown in f. Filament lengths were scaled to the rod length, 32 nm. False color bar: Filament count at each time point.
