Fig. 2: PilA and cluster_3 minor pilins interact.
From: PilY1 and minor pilins form a complex priming the type IVa pilus in Myxococcus xanthus
a BACTH analysis of PilA and cluster_3 minor pilin interactions. T25 and T18 were fused to the N-terminus of the indicated mature, full-length proteins. Positive control (plus) in upper left corner, T25-Zip + T18-Zip; T18 and T25, negative control (minus). Full-length AglQ fused to T25 and T18 served as a specificity control. Left panel, representative images of E. coli BTH101 expressing the indicated protein fusions. For every tested interaction pair, four clones were tested with similar results. Middle panel, relative activity of β-galactosidase shown as mean ± standard deviation (n = 4) for the pairs on the left. Right panel, schematic summarizing observed interactions. b Fractionation of BTH101 E. coli cells expressing the indicated T18 fusions. Cleared cell lysates were fractionated into soluble (S) and membrane (M) fractions. αN N-terminal α-helix in mature pilins, TM trans-membrane helix in AglQ. Proteins from the same number of cells were loaded per lane. Right panel, schematics of domain organization and calculated molecular sizes of the corresponding proteins.
