Fig. 8: CCR2 activity assays in yeast. | Nature Communications

Fig. 8: CCR2 activity assays in yeast.

From: Tailoring poplar lignin without yield penalty by combining a null and haploinsufficient CINNAMOYL-CoA REDUCTASE2 allele

Fig. 8

The relative activity of the mutant P. alba CCR2 protein (as present in CCR2(−/*) line 12) was determined in yeast. a Principle of the yeast feeding assay. Yeast cultures were engineered to express 4CL and the wild-type P. alba CCR2 protein or mutated P. alba CCR2 protein (as present in CCR2(−/*) line 12). After feeding the yeast cultures with ferulic acid, the activity of the respective CCR2 protein was judged based on the production of coniferaldehyde (the product of CCR2, peak 1), coniferyl alcohol (peak 2), and dihydroconiferyl alcohol (peak 3). See Supplementary Fig. 12 for the spectra of peaks 1–3. b GC-MS chromatograms of an authentic coniferaldehyde standard and extracts from ferulic acid-fed yeast cells expressing 4CL in combination with either an empty vector (EV), mutant P. alba CCR2 or wild-type P. alba CCR2. The results shown are representative of five biologically independent samples.

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