Fig. 4: Kinetics of 60S subunit joining and the subsequent transition into elongation in experiments performed with mis-acylated tRNAiMet. | Nature Communications

Fig. 4: Kinetics of 60S subunit joining and the subsequent transition into elongation in experiments performed with mis-acylated tRNAiMet.

From: Structural basis for the transition from translation initiation to elongation by an 80S-eIF5B complex

Fig. 4

The cumulative probability distributions of the dwell times for 60S joining (a) and the transition to elongation (b) from experiments performed at 3 mM free Mg2+ and 20 °C with the model mRNA and unlabeled eIF5B. Data points were fitted to a single-exponential equation with the mean times reported in (c). Errors represent 95% confidence intervals, and the number of molecules analyzed (n) are listed in (c).

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