Fig. 5: mRNA nucleotides at positions −1/−3 play a key role in later stages of initiation. | Nature Communications

Fig. 5: mRNA nucleotides at positions −1/−3 play a key role in later stages of initiation.

From: Structural basis for the transition from translation initiation to elongation by an 80S-eIF5B complex

Fig. 5

a Overall view of the 80S/Met-tRNAiMet/eIF5B complex in an orientation centered on the mRNA. b Detailed view of the small ribosomal subunit P site with experimental cryo-EM density shown. The Met-tRNAiMet ASL is in green, the start codon gray, and ribosomal bases of the small subunit yellow. c Overall view of the P and E sites of the small ribosomal subunit. Six bases corresponding to the start codon of the mRNA and three preceding bases corresponding to nucleotides −1 to −3 showed unambiguous densities and could be modeled (gray). The final refined model with labeled components is shown. d Simplified schematic view of the conformation adopted by the start codon in the P site and the preceding −1 to −3 bases in the E site in the presence of eIF5B.

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