Fig. 5: Lineage tracing using Ngn3 promoter confirms contemporary specification of α and β-cell lineages.
From: Tracing the cellular basis of islet specification in mouse pancreas
a Experimental schedule for Ngn3-CreER lineage tracing at non-clonal levels of induction. b Wide-field section of tissue showing typical labelling efficiency based on 3 experiments (and >10 images) from E12.5 to P14 tracing with labelled cells marked by GFP. c Quantification of the ratio of glucagon+ to insulin+ cells on thin sections from islets as a whole and from labelled (GFP+) cells from E12.5 to P14 and E15.5 to P14 tracings (n = 83 and n = 81 islets, respectively, from N = 3 mice). Error bars show mean ± SD. Representative images of GFP+ cells within islets based on 3 experiments (and >25 images each) traced d from E12.5 to P14 and e from E15.5 to P14. Insulin is red and glucagon is white for (b, d, e). f Summary of the key events in islet formation based on current findings: endocrine progenitor cell commitment, stochastic progenitor expansion and local egression from ducts, islet sublineage restriction and islet fusion, sublineage expansion and islet fission, and continuing β-cell sublineage expansion. Source data provided as Source Data file.
