Fig. 2: Metastable hybridization-based DNA information encoding.
From: Metastable hybridization-based DNA information storage to allow rapid and permanent erasure
a Preparing encoded messages. DNA oligos encoding the intended messages (cyan) are hybridized with truth marker oligos (green). In a separate solution, DNA oligos encoding noise messages (brown) are hybridized with false marker oligos (red). The two solutions are then mixed to form the information solution. b Reading the information solution. DNA polymerase and dNTPs are added to information solution, and the truth markers are extended to copy the intended messages. The false markers (red) have a three C3 functionalization and are unable to be extended. The mixture of extended truth markers is then amplified by PCR to generate a library encoding the true messages, which can then be read by NGS. c Experimental characterization of truth marker binding stability via polyacrylamide gel electrophoresis. Shown in the bottom panel is a composite of two images of the same gel scanned using different fluorescence filter sets, with the FAM image false colored in green, and the ROX image false colored in red. Lanes 1 and 2 show the intended message prehybridized to the truth marker and the noise DNA prehybridized to the false marker, respectively. Lanes 3 and 4 show the intended message prehybridized to the false marker and the noise DNA prehybridized to the truth marker. Lanes 5–9 show the mixture of the species in Lanes 1 and 2 incubated for different amounts of time at different temperatures. See Supplementary Note 2 for further analysis details.
