Fig. 5: Metastasis-capable cells in multiple sites within the prostate and perineural regions in A32. | Nature Communications

Fig. 5: Metastasis-capable cells in multiple sites within the prostate and perineural regions in A32.

From: Prostate cancer evolution from multilineage primary to single lineage metastases with implications for liquid biopsy

Fig. 5

The prostate (center) was divided into 13 slices (black lines) and three regions were sampled, two from slice 10, and one from slice 6. The purple circle denotes the region that was fresh frozen and sequenced as sample c and the red circles denote regions that were ethanol/methanol fixed and paraffin embedded. Histology slides shown to left and right, with microdissected regions highlighted in cyan with adjacent sequencing sample letters. The lower pane shows the phylogenetic tree of the intra-prostatic evolution, with numbers in the circles corresponding to subclonal clusters as derived in Supplementary Fig. 9. Lines joining the miscrodissected regions to a subclonal cluster indicate the final subclone observed in each region; these are designated as high (CCF > 0.5) and low (CCF < 0.5) proportion.

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