Fig. 6: Subclones in tissue samples can be identified in DNA obtained from liquid biopsy. | Nature Communications

Fig. 6: Subclones in tissue samples can be identified in DNA obtained from liquid biopsy.

From: Prostate cancer evolution from multilineage primary to single lineage metastases with implications for liquid biopsy

Fig. 6

Plots showing the proportion of single nucleotide variants corresponding to each subclone (numbered on x-axis) that are observed in each sample (letters on y-axis). The area of the circle is proportional to the cancer cell fraction (CCF) of the subclone, with CCF of 1 found in the leftmost (truncal) nodes of the subplot. Anatomical location of tissue samples are also given in the sample label, and colored as in the legend. Body fluid samples are denoted by the time when the sample was taken (Aut = autopsy, dptd = the number of days prior to death), and the type of fluid (Serum; Plasma; blood, either Clotted or Whole; Cerebrospinal fluid (CSF)). Sample locations in bold are those that contain lineages represented in liquid biopsy at the highest CCF. Tissue samples in black boxes were polyclonal mixtures and contained similar subclonal CCFs to the boxed body fluid sample. Red boxes denote subclones of interest: star denotes subclones corresponding to a lineage found only in a spinal metastasis and serum, both of which were extracted 4040dptd, and dagger denotes a subclone that is only observed in a subdural metastasis and CSF.

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