Fig. 6: Radiation damage affects the distance between residues in the active site.
From: High-resolution cryo-EM structure of urease from the pathogen Yersinia enterocolitica
a Distances between the Ni2+ ions and selected residues involved in their coordination are plotted against the accumulated exposure. For each reconstruction calculated along the exposure, the model was refined, and distances measured. Dots indicate the average and error bars show ±one standard deviation across five refinement runs with different random seeds. Horizontal dashed line in a–i shows the distance in the model obtained from the full reconstruction with all frames. Vertical dashed lines show approximately the exposure at which the density for charged residues completely vanishes (see Supplementary Movie 2). b Correlation coefficients between distance changes along the exposure for selected residues involved in ion coordination. Distance plots shown in a are indicated with a star. c Average B-factors of selected residues and of the global structure (from five refinement runs) plotted against the accumulated exposure.
