Fig. 4: Autocrine and self-signalling potential in Tabula Muris.

NATMI was run across the 117 cell types identified in the Tabula Muris dataset (at the detection rate threshold of 20%). a Autocrine signalling potential of each cell type in Tabula Muris. Each point corresponds to a cell type, and colours indicate broad lineage classes. X-axis shows the fraction of ligands expressed by a given cell type where the cognate receptor is also expressed in the same cell type. Y-axis shows the reciprocal for the fraction of receptors on a given cell type where the cognate ligand is also expressed. Red lines show the mean fractions of ligands and receptors. b Self-signalling potential of each cell type in Tabula Muris. The dashed line indicates the median number of ligand–receptor pairs co-detected in more than 20% of cells of each cell type (at 10CPM threshold). In c–f, cell-to-cell pairs are classified as autocrine (blue), intra-organ (pink), and inter-organ (green) summary edges. The summary edges are then ranked by summed specificity and the distribution of ranks for each of the three classes shown. Error bars represent standard deviation of ranks. c The distribution of ranks of secreted ligand mediated outgoing edges. d The distribution of ranks of secreted ligand mediated incoming edges. e The distribution of ranks of plasma-membrane ligand mediated outgoing edges. f The distribution of ranks of plasma-membrane ligand mediated incoming edges. The average distributions of autocrine, intra-organ and inter-organ edges through 100× randomised ligand–receptor pairs are shown as dashed lines.