Fig. 5: Mechanical itch depends on GRPR neurons.
From: A spinal neural circuitry for converting touch to itch sensation
a Representative image of c-Fos IHC (green) in the dorsal horn of GrprtdTom (red) mice after von Frey hair stimulation (0.07 g) on the nape skin. Arrowhead indicates c-Fos+/ GrprtdTom + neuron. n = 3 mice. Scale bar, 50 μm. b Quantification of the percentage of GrprtdTom + /c-Fos+ neurons in c-Fos+ neurons in laminae I–IIo. n = 3 mice. c, d Mechanical itch test (c) and CQ itch test (d) after 500 ng of BB-sap treatment. (c two-way ANOVA with Bonferroni post-hoc, *p = 0.018, **p = 0.005, ***p = 0.00001, n = 10 mice per group; d unpaired two-tailed Student’s t-test, ***p = 0.00001, n = 10 mice per group). e, f Representative traces showing one GrprtdTom neuron cannot follow 20 µA/20 Hz stimulation. g Percentage of each types of inputs onto GrprtdTom neurons. Data from five mice, n = 42 neurons. All data are presented as means ± s.e.m. and error bars represent s.e.m. Source data are provided as a Source Data file.
