Fig. 6: GRPR neurons form monosynaptic connections with Tac2 neurons.
From: A spinal neural circuitry for converting touch to itch sensation
a, c Representative IHC images of NKB staining (green) in the cervical spinal cord of Tac2tdTom (red) mice (a) and GrprtdTom (red) mice (c). b, d High power images of the boxed areas in a, c, respectively. Scale bars, 20 μm in a, c; 5 μm in b, d. e, f Schematic of intraspinal injection of TVA-EGFP/RVG virus and RV-dG-dsRed virus. g Representative ISH image of Tac2 (blue) in the cervical spinal cord after RVdG injections. Arrowheads indicate GrpriCre starter neurons (yellow) expressing GFP (green) and dsRed (red). Arrows indicate Tac2+ input neurons (dsRed+ and blue+) targeting starter neurons. n = 3 mice. Scale bar, 100 μm.
