Fig. 8: Increased excitability of Tac2^tdTom neurons of dry skin mice.

a–d Resting membrane potential (RMP) (a, b) and the rheobase of action potential (c, d) for Tac2^tdTom neurons in lamina IIi (a, c) and lamina IIIo (b, d). Control: n = 14 neurons in a, b, 13 neurons in c and 12 neurons in d. Dry skin: n = 17 neurons in a, c, 14 neurons in b and 13 neurons in d. Two-tailed Mann–Whitney test. **p = 0.0041, ns not significant. Data were from three mice per group. e Representative traces for Aβ-evoked APs in Tac2^tdTom neurons in laminae IIi of control mice (left) and dry skin mice (right). f, g Percentage of Aβ-evoked APs in Tac2^tdTom neurons in laminae IIi (f) and IIIo (g) of control and dry skin mice, respectively. Control: n = 24 neurons in f, n = 11 neurons in g; dry skin: n = 22 neurons in f, n = 11 neurons in g, Chi-square test, ***p = 0.0001, ns not significant. Data from three mice per group. h Firing patterns of Tac2^tdTom neurons in control mice (n = 24 neurons) and dry skin mice (n = 22 neurons). Data from five mice per group. i Representative traces for Aβ-eIPSCs in Tac2^tdTom neurons in lamina IIi of control mice (left) and dry skin mice (right). j The amplitude of Aβ-eIPSCs on Tac2^tdTom neurons in lamina IIi of control mice and dry skin mice. ***p = 0.00001, two-tailed unpaired Student’s t-test, n = 18 neurons per group. k Representative traces of Aβ-eIPSCs on Tac2^tdTom neurons in lamina IIi before (control) and after bath application of bicuculline (Bic) and/or strychnine (Stry). Blue arrows indicate stimulation artifacts. Data were from three mice per group. l The amplitude of Aβ-eIPSCs on lamina IIi Tac2^tdTom neurons before (control) and after bath application of bicuculline or strychnine. ***p = 0.0001, two-tailed paired Student’s t-test, n = 15 neurons per group. Data are presented as means ± s.e.m. Error bars represent s.e.m. Source data are provided as a Source Data file.