Fig. 1: The senescence state of fibroblasts determines JMJD3’s effect in reprogramming.
From: JMJD3 acts in tandem with KLF4 to facilitate reprogramming to pluripotency
a Correlation between Ink4a induction and cell proliferation in a serial passaging of MEFs. 2 × 105 MEFs per well of a six-well plate were seeded and cell number was counted at day 3 before each passaging. b RT-qPCR for Jmjd3 and Utx in a serial passaging of MEFs. c RT-qPCR for Ink4a in P2 and P4 MEFs transduced with OSKM and empty vector (Empty) or JMJD3 in medium with or without Vc. d, e Images and numbers of AP+ colonies (left panel) and Oct4-GFP+ colonies (right panel) at day 16 (without Vc, d) or day 11 (with Vc, e) in P2 and P4 MEFs transduced with OSKM and Empty or JMJD3. D day. Scale bar, 200 μm. f AP staining images and colony numbers (AP+ and GFP+) at day 10 in P1 and P3 TTFs transduced with OSKM and Empty or JMJD3 in medium with Vc. g AP staining images and colony numbers (AP+ and GFP+) at day 5 in pre-iPSCs transduced with Empty or JMJD3. Error bars represent the s.e.m. Data are presented as mean ± s.e.m. from n = 3 a–g biologically independent experiments. Statistical analyses were performed using a two-tailed unpaired Student’s t-test d–g, or one-way analysis of variance (ANOVA) followed by a Holm–Sidak multiple comparison test c (*P < 0.05; **P < 0.01; ***P < 0.001). P values: 0.0252, 0.0086, 0.0111, 0.0493 c; 0.0095, 0.0031, 0.0012, 0.042 d; 0.0267, 7.98 × 10−5, 0.0005, 0.0043 e; 0.0119, 0.0018, 0.0024, 0.0344 f; 0.0001 g. Source data are provided as a Source Data file.
