Fig. 3: Comparative omics for genes and proteins involved in the reductive glycine pathway.
From: The reductive glycine pathway allows autotrophic growth of Desulfovibrio desulfuricans
Plots per enzyme represent the log10-fold change in autotrophic condition (H2/CO2/sulphate) versus heterotrophic condition (acetate/H2/CO2/sulphate) and versus heterotrophic growth on lactate as sole energy source (lactate/CO2/sulphate), for both proteome and transcriptome analysis. Long-dashed lines are the transporters involved, short dashes incidate the alternative variant route via serine. Cultures were performed in four biological replicates. Growth conditions were 30 °C and 175 rpm in 250 ml glass bottles containing 100 ml anoxic minimal medium. Abbreviations are described in the legend of Fig. 2, with the addition of: ATP/ADP (adenosine tri/diphosphate); CoA (co-enzyme A); cytred/ox (reduced/oxidised cytochrome); FDHap (formate dehydrogenase, accessory protein); FT (formate transporter); GR_Bb (glycine reductase complex; component B, subunit alpha); NAD(P)H (nicotinamide adenine dinucleotide (phosphate)); SDA (serine dehydratase-like); THF: tetrahydrofolate; ND: not detected. Source data are provided with this paper, and complete transcriptomics and proteomics data can be found in Supplementary Data 4 and 5, respectively.
