Fig. 4: Confirmation of the reductive glycine pathway via 13C-labelled metabolomics.
From: The reductive glycine pathway allows autotrophic growth of Desulfovibrio desulfuricans
13C-labelled carbon signature expected in the serine route (a), glycine route (b) and obtained pattern of D. desulfuricans grown in autotrophic conditions (c) with addition of 13C-formate. Autotrophic cultures (H2/CO2/sulphate) were supplemented with 75 mM sodium 13C-formate (99% 13C, Sigma-Aldrich) and grown in 30 mL bottles in 10 mL anoxic medium, cultivations were performed in biological triplicates. The dotted arrows indicate anaplerotic production of oxaloacetate via pyruvate carboxylase. Gly (glycine); Ser (serine); Ala (alanine); Val (valine); Leu (leucine); Thr (threonine); Pro (proline). Source data are provided with this paper, and data can be found in Supplementary Data 6.
