Fig. 6: Cellular ligands of CD33 on human monocytes are both α2-3 and α2-6 sialosides.
From: A versatile soluble siglec scaffold for sensitive and quantitative detection of glycan ligands
a Staining of U937 cells treated with an α2-3-specific sialiadase (Neu-S) or broadly acting sialiadase (Neu-A) with CD33-Fc pre-complexed with Strep-Tactin-AF647. b Staining of WT and ST6Gal1−/− U937 cells with CD33-Fc pre-complexed with Strep-Tactin-AF647. c CD33-Fc binding to human peripheral blood monocytes treated with Neu-S or Neu-A. d Depiction of the unmasking assay performed using CD33 high-affinity ligand (CD33L, compound 6) displaying liposomes on WT cells or cells treated with neuraminidase. e, f Binding of CD33 ligand-targeted liposomes (red) or naked liposomes (black) to U937 cells (e) or to human peripheral blood monocytes (f). Error bars represent ± standard deviation of three replicates. Statistical significance calculated based on a two-tailed unpaired Student’s T-test.
