Fig. 4: iCAFs promote proliferation of cancer cells. | Nature Communications

Fig. 4: iCAFs promote proliferation of cancer cells.

From: Single-cell RNA sequencing highlights the role of inflammatory cancer-associated fibroblasts in bladder urothelial carcinoma

Fig. 4

a Heatmap of the area under the curve (AUC) scores of TF motifs estimated per cell by SCENIC. Shown are top five differentially activated motifs in iCAFs and mCAFs, respectively. b tSNE plots of the expression levels of TFs (up) and AUC scores (down). c Dot plot shows the expression level of growth factors across cell types. iCAFs are the major producer of growth factors. d tSNE plot shown the expression level of IGF1. IGF1 is secreted almost only by iCAFs. e High level IGF1 represents poor overall survival in TCGA BLCA cohort. P value was calculated with log-rank test. f FACS sorting strategy of iCAFs. g Co-culture and colony formation experiment showed that iCAFs have pro-proliferation property in vitro (n = 5). Error bar: mean value ± sd. P values were determined by two-side Student’s t test. ***p < 0.001.

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