Fig. 8: NOTCH1 silencing or inhibition suppress CAFs-mediated proliferation potential of SCC13 cancer cells. | Nature Communications

Fig. 8: NOTCH1 silencing or inhibition suppress CAFs-mediated proliferation potential of SCC13 cancer cells.

From: NOTCH1 gene amplification promotes expansion of Cancer Associated Fibroblast populations in human skin

Fig. 8

a, b Representative images and relative quantification of immunofluorescence analysis with anti-PANKERATIN (magenta) and anti-VIMENTIN (cyan) antibodies of SCC13 cells co-cultured at a 1:1 ratio with CAF strains infected with a NOTCH1 silencing lentivirus versus empty vector control for 3 days (a) or treated with DBZ (10 µM dose) versus DMSO for 3 days (b). Graphs show the quantification of large SCC13 cell clusters (>40 cells) per examined field. Scale bar, 200 μm. Values for each field are indicated as dots with mean ± s.d. a One-way ANOVA with Dunnett’s test and b two-tailed unpaired t-test, **p < 0.01, *** p < 0.001, ****p < 0.0001, n(CAF strain) = 3. n(number of fields, per sample) = 10.

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