Fig. 3: CTSD deficiency induces a quiescent cell state in short-term starved tumor cells. | Nature Communications

Fig. 3: CTSD deficiency induces a quiescent cell state in short-term starved tumor cells.

From: Cathepsin D deficiency in mammary epithelium transiently stalls breast cancer by interference with mTORC1 signaling

Fig. 3

a Pictures of Ctsd+/+ and Ctsd−/− PyMT cells cultured for 7–10 days in 10% FCS or 1% FCS medium. Bars, 50 μm. Representative of 5 independent experiments. b Staining for acidic β-galactosidase in 10% FCS and 1% FCS Ctsd+/+ and Ctsd−/− PyMT cells. Bars, 50 μm. Representative of 3 independent experiments. c Quantification of label-retaining cells among 10% FCS and 1% FCS Ctsd+/+ and Ctsd−/− PyMT cells (n = 7 independent experiments; two-sided two-sample t-test). d Amount of Annexin-V-7-AAD−(living) cells among 1% FCS Ctsd+/+ and Ctsd−/− PyMT cells treated with Navitoclax, relative to DMSO control and Ctsd+/+ (n = 3 independent experiments; two-sided one-sample t-test). e Quantification of IL-6 by alphaLISA in cell-conditioned media from 10% FCS and 1% FCS Ctsd+/+ and Ctsd−/− PyMT cells (n = 2 independent experiments). f Relative Cdkn2a/p16 and Cdkn1a/p21 expression determined by RT-PCR in 10% FCS and 1% FCS Ctsd+/+ and Ctsd−/− PyMT cells (n = 3 independent experiments; two-sided two-sample t-test). Bar charts show all data points with mean + SD and p-value. Source data are provided as a Source Data file.

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