Fig. 8: CTSD-deficient tumor cells having escaped quiescence activate tumorigenic CREB. | Nature Communications

Fig. 8: CTSD-deficient tumor cells having escaped quiescence activate tumorigenic CREB.

From: Cathepsin D deficiency in mammary epithelium transiently stalls breast cancer by interference with mTORC1 signaling

Fig. 8

a Phospho-kinase antibody arrays with lysates from Ctsd+/+ and Ctsd−/− PyMT cells cultured for 7–10 days (1% FCS) or for ≥8 weeks (1% FCS LT) in 1% FCS medium. Changes in phosphorylation are plotted as log2 fold change (FC) for indicated comparisons (n = 2 independent experiments). b, c Transcriptome analysis of 1% FCS and 1% FCS LT Ctsd+/+ and Ctsd−/− PyMT cells (n = 3 independent experiments). b Change in expression of significantly regulated genes of the CREB gene set for indicated comparisons. Red dashed lines, borders for gene up- and downregulation (|log2 FC | > 1). c Clustering analysis of the log2 FC of CREB genes with significant upregulation in Ctsd−/− 1% FCS LT versus 1% FCS PyMT cells for indicated comparisons shown as heatmap. Source data are provided as a Source Data file.

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