Fig. 2: JEV-resistance screen in PK-15 cells using the porcine genome-scale CRISPR/Cas9 knockout cell collection. | Nature Communications

Fig. 2: JEV-resistance screen in PK-15 cells using the porcine genome-scale CRISPR/Cas9 knockout cell collection.

From: CRISPR screening of porcine sgRNA library identifies host factors associated with Japanese encephalitis virus replication

Fig. 2

a Workflow and screening strategy for the CRISPR/Cas9 screen. b, c Scatter plots comparing sgRNA targeting sequences frequencies and extent of enrichment vs. the noninoculated control mutant cell pool for the third or fourth (c) rounds of JEV screens after challenge. Counts_JEV3rd and Counts_JEV4th represent the average values of the read counts from paired-end sequencing, respectively. d, e Venn diagrams showing the overlapping enrichment of specific sgRNAs targeting sequences in the third or fourth rounds of JEV screens after challenge. For d, among the top 0.1% of averaged reads for the sgRNAs; for e, among the top 0.5%. f KEGG pathway enrichment analyses for the top 0.5% ranked sgRNA targets from the third and fourth JEV challenge rounds. JEV, Japanese encephalitis virus; MOI, multiplicity of infection; sgRNA, small guide RNA; JEV3rd, the third challenge round of JEV screening; JEV4th, the fourth challenge round of JEV screening; FACS, Fluorescence-activated cell sorting; JEV-RP9, JEV, genotype 3, strain RP9. Source data are provided as Supplementary Data files.

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