Fig. 3: Significant enrichment of specific sgRNAs targeting 10 genes involved in HSPGs synthesis and metabolic pathways. | Nature Communications

Fig. 3: Significant enrichment of specific sgRNAs targeting 10 genes involved in HSPGs synthesis and metabolic pathways.

From: CRISPR screening of porcine sgRNA library identifies host factors associated with Japanese encephalitis virus replication

Fig. 3

a Venn diagram showing the overlapping enrichment of specific sgRNA targeted genes identified in the JEV screen of the cell knockout collection, HSPGs biosynthesis and metabolism, and GAG metabolism pathways. b Enrichment of specific sgRNAs targeting 10 genes involved in HSPGs synthesis and metabolic pathways. Only the top 0.5% of sgRNA was counted. c Schematic diagram, adapted from Tanaka et al. 28, showing the various classes of chemical components comprising HSPGs with known enzymes for their biosynthesis. Red indicates targeted genes identified in this study. d Schematic diagram, adapted from Blondel et al. 34, showing the known components and localization information for the sulfurylation modifications known to occur for some HSPGs. Red indicates targeted genes identified in this study. JEV, Japanese encephalitis virus; HS, Heparan sulfate; GAG, Glycosaminoglycan; HSPGs, heparan sulfate proteoglycans; PAPS, 3’-Phosphoadenosine-5’-phosphosulfate; JEV3rd, the third challenge round of JEV screening; JEV4th, the fourth challenge round of JEV screening. Source data are provided as a Supplementary Data file.

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