Fig. 7: A proposed model for JEV entry and replication in porcine cells. | Nature Communications

Fig. 7: A proposed model for JEV entry and replication in porcine cells.

From: CRISPR screening of porcine sgRNA library identifies host factors associated with Japanese encephalitis virus replication

Fig. 7

The JEV-infection cycle starts with binding to co-factors HSPGs, and/or unknown cellular receptors, followed by viral entry to enable replication. The EMC complex protein (EMC3 and EMC6) and CALR calcium-binding protein of the ER lumen are involved in JEV replication of host cells. Subsequently, the JEV RNA genome is replicated, viral particles are matured and packaged, and are released from cells. JEV, Japanese encephalitis virus; HSPG, heparan sulfate proteoglycan; PAPS, 3′-Phosphoadenosine-5′-phosphosulfate; PAP, 3′-phosphoadenosine-5′-phosphate; ATP, Adenosine triphosphate; ADP, Adenosine diphosphate; APS, Adenosine 5′ phosphosulfate; PPI, pyrophosphate; ER, endoplasmic reticulum; ERAD, endoplasmic reticulum-associated protein degradation; EMC, endoplasmic reticulum membrane protein complex.

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