Fig. 2: Dissection of MF synapse CSP composition. | Nature Communications

Fig. 2: Dissection of MF synapse CSP composition.

From: Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer

Fig. 2

a Protein functional classes represented in the group of CSPs identified in isolated MF synaptosomes. b Occurrence of protein domains among identified CSPs. c SynGO cellular component analysis of CSP genes (75) and respective CSPs (77). Discrepancy between gene number and CSP number is related to NRXN isoforms. Proteins with unknown function in the brain are highlighted in bold with an asterisk: APMAP, BRINP2, FAM171A2, and IgSF8. d Validation of 15 CSPs in sorted MF synaptosomes by western blot, including BRINP2 and IgSF8, CSPs without a known function in the brain. e Confocal images of P28 mouse hippocampal sections immunostained for 15 CSPs showing a striking laminar distribution to the SL. Of the CSPs without a known function in the brain, IgSF8 showed strong labeling in the MF pathway. Source data are provided as a Source data file. Scale bar in e 200 μm.

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