Fig. 5: Balancing cell growth with biosynthesis of N-acetylneuraminic acid (NeuAc). | Nature Communications

Fig. 5: Balancing cell growth with biosynthesis of N-acetylneuraminic acid (NeuAc).

From: Titrating bacterial growth and chemical biosynthesis for efficient N-acetylglucosamine and N-acetylneuraminic acid bioproduction

Fig. 5

a NeuAc biosynthetic pathways in B. subtilis. G6P, Glucose-6P; F6P, Fructose-6P; FBP, Fructose-1,6-2P; GAP, glyceraldehyde-3-P; PEP, phosphoenolpyruvate; GlcNAc, N-acetylglucosamine; ManNAc, N-acetylmannose. b Fermentation efficiencies of engineered B. subtilis strains at different O-methyl-L-tyrosine (OMeY) concentrations. c Amplifying gene age and neuB from plasmid pP43NMK and inserting into pBUA-P224 under the control of different promoters. d NeuAc production of seven single-plasmid NeuAc biosynthesis strains. e Fermentation efficiencies of NAB-SpP566 and NABd8-SpP566 in a 3-L fermenter. All the data are expressed as the mean ± SD from three (n = 3) biologically independent replicates. Source data underlying (b), (d), (e) are provided as a Source data file.

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